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KMID : 0364819880260030173
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Korean Journal of Microbiology
1988 Volume.26 No. 3 p.173 ~ p.180
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Transter of RP4::Mu cts and RP4::mimi-Mu from E. coli to Pseudomonas sp.
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Ko, Yun-Won, Huh, Youn-Ju/고윤원
Lee, Yung-Nok/허연주
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Abstract
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1
Cbromosomal gene transferable hybrid plasmids, RP4::Mu cis and RP4::mini-Ma, were transferred by conjugation from E. coil to Pseudomonas strains. In order to use for recipient cuts of RP4:: Mu cts and RP4:: mini-Mu, pbum d-free Psadomonar strains were characterised for their antibiotic resistance, aromatic hydrocarbon utility and degradation patterns of chlorinated berbidde. Transfer frequencies of RP4:: mini-Ma exhibited about 10-2 to 10-4, while those of RP4:: Mu cts exhibited very low value of lo´7 in recipients tested except Psadomonas aerwgnosa KU557. Existaace of hybrid plasmids In Pseudomonas transcoijugants were Identified by their antibiotic resistance and agarose gel dectrophoresls. In case of RP4:: Ma cts trancon ju`aats it was also confirmed by demoustrating that they were capable of releasing phnge and form rag plaques at 43°C. Plaque forming unit of the transcoojugants was about 105. It was shown by the stability test that RP4:: Mu cts and RP4:: ml lMu In Pseudomonas were relatively stable.
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